OncoStrands® Promoter Methylation > DNA Laboratories Staging Site

Specimen Requirements
Clinical usage	PCR-based methylation tests are used to inform and help predict patient outcomes, as well as serve as a guide for preventive measures, such as surveillance and risk reduction strategies, in individuals with an increased risk of developing cancer. – MLH1: Detection of methylation of the MLH1 gene promoter on FFPE, adjunct to MSI, MMR IHC, and for colon or endometrial tumours demonstrating MSI-H and loss of MLH1 protein expression. – MGMT: Detection of methylation of MGMT gene promoter on FFPE, for prognostic and predictive value for glioblastoma patients.
Type of sample	– Formalin-fixed paraffin-embedded (FFPE) Tissue/Cell Block (preferred) or – 10 x 5µm unstained slides of FFPE tissue mounted on uncoated slides + 1 H&E stained slide – 10 x 5µm normal tissue slides (If available for MLH1 promoter methylation test)
Surface Area & Tumour Purity	Minimum surface area of 5 x 5 mm2 per slide. If the tissue area is smaller, please provide additional 5-10 5µm unstained slides. At least 10% tumour purity per slide.
Storage & transportation	Please keep at room temperature. Do not freeze, and keep away from direct sunlight.
Turnaround Time (TAT)	12 working days (No additional day if add-on)

Specimen Requirements

Clinical usage

PCR-based methylation tests are used to inform and help predict patient outcomes, as well as serve as a guide for preventive measures, such as surveillance and risk reduction strategies, in individuals with an increased risk of developing cancer.

– MLH1: Detection of methylation of the MLH1 gene promoter on FFPE, adjunct to MSI, MMR IHC, and for colon or endometrial tumours demonstrating MSI-H and loss of MLH1 protein expression.
– MGMT: Detection of methylation of MGMT gene promoter on FFPE, for prognostic and predictive value for glioblastoma patients.

Type of sample

– Formalin-fixed paraffin-embedded (FFPE) Tissue/Cell Block (preferred) or
– 10 x 5µm unstained slides of FFPE tissue mounted on uncoated slides + 1 H&E stained slide
– 10 x 5µm normal tissue slides (If available for MLH1 promoter methylation test)

Surface Area & Tumour Purity

Minimum surface area of 5 x 5 mm2 per slide. If the tissue area is smaller, please provide additional 5-10 5µm unstained slides. At least 10% tumour purity per slide.

Storage & transportation

Please keep at room temperature. Do not freeze, and keep away from direct sunlight.

Turnaround Time (TAT)

12 working days (No additional day if add-on)